Materials

Supplementary information for "Mapping developmental maturation of inner hair cell ribbon synapses in the apical mouse cochlea" by Michanski et al., PNAS

  • SI Appendix Movie S1: FIB-SEM visualization of the basolateral compartment of a P15 inner hair cell (MP4 video).
    Movie scanning through a representative FIB-SEM z-stack of a P15 IHC, showing 3D reconstruction of the basolateral IHC contour, part of the nucleus, mitochondria, innervating nerve fibers, synaptic ribbons and their corresponding SVs. The movie starts at the nuclear region and extends towards the base of the cochlear IHC.
  • SI Appendix Movie S2: FIB-SEM z-stack of the supranuclear part of two P9 inner hair cells (MP4 video).
    Movie scanning through a FIB-SEM z-stack of the supranuclear part of two neighboring P9 IHCs, shown in Fig. S7K. No ribbon precursors or ribbons could be observed in this dataset. Scale bar: 5 μm.

CalC script for the simulation of AZ Ca2+ spread in IHCs

Routines for the analysis of whole-cell capacitance measurements

  • Custom-written Igor pro routines for the analysis of depolarization-evoked capacitance changes from Patch Clamp data (Pulse or Patchmaster format).
    Igor procedure file v. 2.2.4.2, last updated 23.08.2017
    For questions, please contact Jakob Neef.

Modes and Regulation of Endocytic Membrane Retrieval in Mouse Auditory Hair Cells

  • Supplementary information for "Modes and Regulation of Endocytic Membrane Retrieval in Mouse Auditory Hair Cells" by Neef et al., J Neurosci 2014

A Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosis

  • A Probing the functional equivalence of otoferlin and synaptotagmin 1 in exocytosis", by Reisinger et al.,  J. Neuroscience 2011. (AVI format)

Materials for on-cell capacitance measurements (if interested contact Andreas Neef)

  • Guide to low noise on-cell capacitance measurements using the EPC-10
  • Patchmaster files (for recent files and program version visit the download section at www.heka.com) On Cell CM
  • Igor Pro Template for analysis of on-cell lock-in experiments, inlcuding example data Software LockIn Analysis IGOR, PXT format

Materials for ASSR measurements

  • Matlab-routine for recording the modulation transfer function and the ASSR threshold detection ASSR-Matlab-routine

Materials for non-stationary fluctuation analysis of whole cell capacitance measurements (if interested contact Andreas Neef)

Material for estimation of synapse distribution in 3D image stacks

  • Custom-written matlab routines for the estimation of the cellular synapse distribution in 3D image stacks. Matlab-routines
    For questions, please contact Gerhard Hoch.
backtotop